PlanetCatfish.com

Hi Jools,

I was trying to make a post in the Science News forum and I received this error:

Internal Server Error
The server encountered an internal error or misconfiguration and was unable to complete your request.

Please contact the server administrator at webmaster@planetcatfish.com to inform them of the time this error occurred, and the actions you performed just before this error.

More information about this error may be available in the server error log

... and yet, I can post this here.

Maybe it's something in the text I'm uploading?

Cheers, Eric

Here is the title and text:

Genetic diversity, spatial connectivity, and population structure of Wallago attu

Kumar, A., Negi, N., Yadav, N., Badola, R., Hussain, S.A. & S.K. Gupta. (2024). Genetic diversity, spatial connectivity, and population structure of Asian silurid catfish Wallago attu (Bloch and Schneider, 1801) in the Ganga River System: insights from mitochondrial DNA analysis. Molecular Biology Reports, 51(1), p.378.
Abstract
Background The Ganga River System (GRS) is a biodiversity hotspot, its ecological richness is shaped by a complex geological history. In this study, we examined the genetic diversity, spatial connectivity, and population structure of the Asian Silurid catfish, Wallago attu, across seven tributaries of the GRS.
Methods and results We employed three mitochondrial DNA (mtDNA) regions: cytochrome c oxidase subunit I (COXI), cytochrome b (Cyt b), and control region (CR). Our comprehensive dataset encompassed 2420 bp of mtDNA, derived from 176 W. attu individuals across 19 sampling sites within the seven rivers of GRS. Our findings revealed high gene diversity (Hd:0.99) within W. attu populations. Analysis of Molecular Variance (AMOVA) highlighted that maximum genetic variations were attributed within the populations, and the observed genetic differentiation among the seven populations of W. attu ranged from low to moderate. Network analysis uncovered the presence of three distinct genetic clades, showing no specific association with seven studied rivers. Bayesian skyline plots provided insights into the demographic history of W. attu, suggesting a recent population expansion estimated to have occurred approximately 0.04 million years ago (mya) during the Pleistocene epoch.
Conclusions These results significantly enhance our understanding of the genetic diversity and spatial connectivity of W. attu, serving as a vital foundation for developing informed conservation strategies and the sustainable management of this economically valuable resource within the Ganga River System.
Keywords Wallago attu, Mitochondrial DNA, Genetic diversity, Population genetic structure, Ganga river system

I figured it out. It's the ITALICS tags.

My original text had the CLOG tag, a QUOTE tag and ITALICS tags. With all these tags, I got the error messages. With no tags, the text uploaded. I added back the QUOTE tag and it still saved. I added back the CLOG tag and it still saved.

Then I added back one set of ITALICS tags and it crashed again with the error message. Don't recall ever getting that error before.

Cheers, Eric

Here is the title and text and QUOTE and CLOG tags, no ITALICS tags:

Genetic diversity, spatial connectivity, and population structure of Wallago attu

Kumar, A., Negi, N., Yadav, N., Badola, R., Hussain, S.A. & S.K. Gupta. (2024). Genetic diversity, spatial connectivity, and population structure of Asian silurid catfish Wallago attu (Bloch and Schneider, 1801) in the Ganga River System: insights from mitochondrial DNA analysis. Molecular Biology Reports, 51(1), p.378.

Abstract
Background The Ganga River System (GRS) is a biodiversity hotspot, its ecological richness is shaped by a complex geological history. In this study, we examined the genetic diversity, spatial connectivity, and population structure of the Asian Silurid catfish,

Wallago attu

, across seven tributaries of the GRS.
Methods and results We employed three mitochondrial DNA (mtDNA) regions: cytochrome c oxidase subunit I (COXI), cytochrome b (Cyt b), and control region (CR). Our comprehensive dataset encompassed 2420 bp of mtDNA, derived from 176 W. attu individuals across 19 sampling sites within the seven rivers of GRS. Our findings revealed high gene diversity (Hd:0.99) within W. attu populations. Analysis of Molecular Variance (AMOVA) highlighted that maximum genetic variations were attributed within the populations, and the observed genetic differentiation among the seven populations of W. attu ranged from low to moderate. Network analysis uncovered the presence of three distinct genetic clades, showing no specific association with seven studied rivers. Bayesian skyline plots provided insights into the demographic history of W. attu, suggesting a recent population expansion estimated to have occurred approximately 0.04 million years ago (mya) during the Pleistocene epoch.
Conclusions These results significantly enhance our understanding of the genetic diversity and spatial connectivity of W. attu, serving as a vital foundation for developing informed conservation strategies and the sustainable management of this economically valuable resource within the Ganga River System.

Keywords Wallago attu, Mitochondrial DNA, Genetic diversity, Population genetic structure, Ganga river system

I'm not sure the tags would do this. It is entirely repeatable if you take out and put in the italics tags?

Cheers,

Jools

Genetic diversity, spatial connectivity, and population structure of Wallago attu

Kumar, A., Negi, N., Yadav, N., Badola, R., Hussain, S.A. & S.K. Gupta. (2024). Genetic diversity, spatial connectivity, and population structure of Asian silurid catfish Wallago attu (Bloch and Schneider, 1801) in the Ganga River System: insights from mitochondrial DNA analysis. Molecular Biology Reports, 51(1), p.378.

It works with this much text, but when I put the whole thing in, I still get the error

Can there be too many tags in a single post now?

Ok, so this one is not on me! The below is from the error log [note to self] and it shows the server security configuration causing the issue. Will raise with our server hosts.

Cheers,

Jools

Test:

Here is the title and text:

Genetic diversity, spatial connectivity, and population structure of Kumar, A., Negi, N., Yadav, N., Badola, R., Hussain, S.A. & S.K. Gupta. (2024). Genetic diversity, spatial connectivity, and population structure of Asian silurid catfish Wallago attu (Bloch and Schneider, 1801) in the Ganga River System: insights from mitochondrial DNA analysis. Molecular Biology Reports, 51(1), p.378.

Abstract
Background The Ganga River System (GRS) is a biodiversity hotspot, its ecological richness is shaped by a complex geological history. In this study, we examined the genetic diversity, spatial connectivity, and population structure of the Asian Silurid catfish, Wallago attu, across seven tributaries of the GRS.

Methods and results
We employed three mitochondrial DNA (mtDNA) regions: cytochrome c oxidase subunit I (COXI), cytochrome b (Cyt b), and control region (CR). Our comprehensive dataset encompassed 2420 bp of mtDNA, derived from 176 W. attu individuals across 19 sampling sites within the seven rivers of GRS. Our findings revealed high gene diversity (Hd:0.99) within W. attu populations. Analysis of Molecular Variance (AMOVA) highlighted that maximum genetic variations were attributed within the populations, and the observed genetic differentiation among the seven populations of W. attu ranged from low to moderate. Network analysis uncovered the presence of three distinct genetic clades, showing no specific association with seven studied rivers. Bayesian skyline plots provided insights into the demographic history of W. attu, suggesting a recent population expansion estimated to have occurred approximately 0.04 million years ago (mya) during the Pleistocene epoch.

Conclusions These results significantly enhance our understanding of the genetic diversity and spatial connectivity of W. attu, serving as a vital foundation for developing informed conservation strategies and the sustainable management of this economically valuable resource within the Ganga River System.

Keywords
Wallago attu, Mitochondrial DNA, Genetic diversity, Population genetic structure, Ganga river system

It's working!!!

Cheers,

Jools