Building on some of the efforts I've seen here and elsewhere, for the last six months or so I've been working towards getting Otocinclus to spawn in my aquariums.
I have two 20Ls set up at the moment, with only otos in both. Both dirt substrate, heavily planted, ph~6.5-7 and very soft water (KH/GH<3).
The high flow tank (the "blue" tank, named for the colour of the antivibration mat it sits on) has only been going for a month or so. Solely huaorani occupants, about 13 at present. Mix of genders and ages, with some juveniles and some fully grown. Substrate has a layer of peat under the sand cap, over the soil mix.
Set up for a circling current around the centre driftwood island, about 300GPH turnover. The otos love this tank, and are much more active than the other one I'll describe. Especially after the weekly water change (25%, RO), they play and jet all over the tank. Their tails are almost always fully spread, which seems a good sign, and they're not at all timid about people going up to view the tank.
https://www.youtube.com/watch?v=3krhUG7dv0M
http://youtu.be/39GuSsiCpRE
On the "green" tank, there are two Aquaclear 50s providing filtration, but no directed current. One filter runs peat and foam, the other biomax and foam. About 20 odd otos, mix of huaorani, vittatus and affinis. Huge range of ages, from really little to fully grown.
This tank has a more densely planted mix, and is over a year old right now. The occupants are quite timid, and dash away at any movement in the room.
http://youtu.be/xslW_3lByTk
I've tried to replicate bio-appropriate water conditions, but so far no spawning. I even use a "rain plate" to deliver the water change water, which is a few degrees cooler than the tank temps (76-77F).
http://youtu.be/GlFNhNZd6go
Both tanks get lots of veggies, which are always polished off within a day or at most two. I supplement with algae rocks which are cultured in another tank.
Any suggestions on what I can do or change? Going to set up another high flow tank, since they seem much happier there. Both tanks rarely have any non-Darwinian losses (ie, oto gets wedged and dies behind filter, in pump, behind plant etc)